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Using antibodies a laboratory manual by Edward Harlow

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Published by Cold Spring Harbor Laboratory Press in Cold Spring Harbor, N.Y .
Written in English


  • Immunoglobulins -- Laboratory manuals.,
  • Immunochemistry -- Laboratory manuals.

Book details:

Edition Notes

StatementEd Harlow, David Lane.
ContributionsLane, David, 1952-
LC ClassificationsQR186.7 .H37 1999
The Physical Object
Paginationxiv, 495 p. :
Number of Pages495
ID Numbers
Open LibraryOL380586M
ISBN 100879695447, 0879695439
LC Control Number98042802

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This second edition of Making and Using Antibodies: A Practical Handbook provides clear guidance on all aspects of how to make and use antibodies for research along with their commercial and industrial applications. Keeping pace with new developments in this area, all chapters in this new edition have been revised, updated, or expanded. About this title. Few technical manuals have become standards in biomedicine. Antibodies: A Laboratory Manual, by Ed Harlow and David Lane, has had that status for a decade. Now there is a new and even higher standard Using Antibodies: A Laboratory Manual. Harlow and Lane have completely revised their guide to the use of immunoglobulin reagents in the : Paperback. Using Antibodies: A Laboratory Manual, Volume Edward Harlow, David Lane No preview available - /5(1). The book covers such topics as hybridoma technology, antibody engineering, the application of antibodies to assay development and blotting techniques, the diagnosis of microbial organisms using antibodies, and the use of antibodies for the study of cells and tissues. Each chapter comes with references and general laboratory protocols.

As Dr. Greenfield notes in his preface to this second edition: "The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. Everything we needed to know to make a monoclonal antibody was all there, neatly packaged in an easy-to-understand second edition is intended to provide the . Therapeutic antibodies can be derived from a variety of sources such as mice, rats, rabbits, non-human primates, or humans, and then engineered to be as human as possible, using information based on natural human antibodies, the genes that produce them, and even B cell biology. Antibody Sequencing: GenScript’s advanced Antibody Sequencing technology offers fast and professional sequencing services for your monoclonal antibodies. Assays: GenScript has developed several cell-based ADCC/CDC functional assays to profile the efficacy and potency of your therapeutic antibodies using proprietary recombinant effector cells. antibody identification tests. 3. Explain heterozygosity and homozygosity as they apply to antibody identification. 4. List allelic pairs in the following blood group systems: Rh, Duffy, Kidd, MNSs. 5. Given patient test results, work through the antibody identification process.

New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present descriptions of basic methods for making and using antibodies and describe basic laboratory techniques in detail. Description. Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology. The Third Edition of this well established book meets the needs of both newcomers to the area and experienced researchers, by providing an integrated treatment of both the production .   Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols. Written and edited by an elite team of scientiCited by: Antibodies book. Read 90 reviews from the world's largest community for readers. A novel based on the Emmy Award-winning television series created by Chr /5(90).